ETAD Methods

Since its foundation, ETAD has accompanied its activity of monitoring ecological and toxicological issues on colorants with the development of corresponding methods for the determination of substances and properties of concern.

Thanks to the expertise of ETAD’s member companies, it has been possible to develop methods which suit the specific chemicophysical properties of dyes and organic pigments and allow a correct assessment of their impact on health and the environment.

The complete list of ETAD Methods can be read below or downloaded as a pdf file.

For further information please contact us.

04/04/2009 - ETAD Methods ETAD Ecological Methods

No. 101	A simple method of determining the biodegradability of products and contaminants in waste water. Static method (R. Zahn and A. Wellens), 1975.
No. 102	Continuous activated sludge test. Modified OECD-confirmatory test for the determination of biological elimination. (Also known under the recent designation of OECD dynamic simulation test), 1975.
No. 103	A screening test for the assessment of the possible inhibitory effect of a chemical substance on aerobic wastewater bacteria, 1979.
No. 104	Method of evaluation of inhibition of microbial respiratory activity of the microorganisms of activated sludges and the microorganisms of rivers, 1979.
No. 105	A screening test for assessing the primary anaerobic biodegradability of water-soluble dyestuffs, 1982.

04/04/2009 - ETAD Methods ETAD Analytical Methods (PCBs)

A 4003-214-3/1979	Determination of polychlorobiphenyls (PCBs) in copper phthalocyanine, 1979.
A 4003-218-1/1986	Determination of polychlorobiphenyls (PCBs) in copper phthalocyanine using reduction to biphenyl, 1986.
A 4003-219-9/1979	Determination of polychlorobiphenyls (PCBs) typically present in trace amounts in 3,3'-dichlorobenzidine-based pigments, 1979.
A 4003-222-8/1981	Determination of decachlorobiphenyl in fully chlorinated phthalocyanines, 1981.
A 4003-223-7/1985	Determination of monochlorobiphenyls in copper phthalocyanine, 1985.
A 4003-224-9/1986	Determination of polychlorinated biphenyls (PCBs) by perchlorination, 1986.
A 4003-3/1989	Guidelines for the determination of inadvertent polychlorinated biphenyls (PCBs) in colorants, 1989.
A 4003-226-12/1987	Determination of the maximum possible level of polychlorobiphenyls (PCBs) and polychloroterphenyls (PCTs) in colorants by perchlorination, 1987.
No 229 NEW	Determination of total content of polychlorinated biphenyls in pigments by dissolution, cleanup and GC/MS, 2011.
M 1/8-1/1987	Isolation of chlorinated biphenyls from colorants insoluble in water and in organic solvents via Soxhlet extraction, 1987.
M 1/9-1/1987	Isolation of lipophilic impurities (e.g., PCBs) from colorants via liquid/liquid extraction, 1987.
M 1/10-1/1987	Isolation and clean-up of polychlorinated biphenyls (PCBs) from colorants via column chromatography, 1987.
M 3/9-8/1990	Determination of PCBs in colorants by combined GC-MS detection, 1990.
M 3/10-8/1990	Experience with Sistovaris and Donges' Method for determination of adventitious PCBs and PCTs in colorants. Report by Analytical Subcommittee, 1990.

04/04/2009 - ETAD Methods ETAD Analytical Methods (general)

No. 201	Method for the determination of α- and β-naphthylamine in C. I. Solvent Brown 1/2,1975.
No. 202	Method for the determination of α- and β-naphthylamine in C. I. Acid Black 7. This method is suitable for water-soluble dyestuffs, 1976.
No. 203	Determination of C. I. Disperse Blue 295 in mirror carp (Cyprinus carpio L.), 1976.
No. 204	The accumulation of C. I. Acid Yellow 222 in the bodies of various types of fish, 1976.
No. 205	Determination of disperse dyes in fish, 1976.
No. 206	Determination of C. I. Basic Yellow 49 in fish, 1976.
No. 207	Determination of the accumulation of C. I. Reactive Red 158 in the bodies of golden orfe, 1976.
No. 208	Determination of the accumulation of C. I. Reactive Blue 121 in fish, 1976.
No. 209	Determination of Direct Black ANBN in the presence of whole fish (goldfish and carp), 1976.
No. 210	The determination of reactive dyes in dilute aqueous solution. Range: 0.1 to 1 ppm, 1976.
No. 211	Accumulation of dye in fish; methods of determination for chromium, nickel and cobalt complex dyestuffs, 1976.
No. 212	Proposed method for the determination of unsulphonated primary aromatic amines in pigments and in solvent-soluble dyestuffs intended for use in food packaging materials, 1979.
No. 212 revised	Determination of unsulfonated primary aromatic amines in pigments and in solvent-soluble dyestuffs intended for use in food packaging. Revision November 1984. This method was approved as DIN 55610 „Prüfung von Pigmenten und lösungsmittellöslichen Farbstoffen. Bestimmung unsulfonierter, primarer aromatischer Amine”
No. 213	Determination of reactive and acid dyes in fish (carp), 1979.
No. 215	Determination of 4-aminobiphenyl in C. I. Solvent Black 5, 1979.
No. 216	Determination of C. I. Acid Green 108 in fish flesh (carp) using high performance liquid chromatography, 1980.
No. 217	Determination of 4-aminobiphenyl and benzidine in C. I. Solvent Black 5 (Nigrosines and Indulines), 1979.
No. 221	Quantification of the limits of “no visible migration" of synthetic organic pigments under conditions of testing using the contact paper method,1980.
No. 225	Determination of Cr(VI) traces in water-soluble Cr(III)-complex dyes by ion-chromatography, 1987.
Supplement to No. 225	An analytical procedure to determine the level of extractable chromium in solvent soluble chromium(III) complex dyestuffs, 1987.
No. 227	Monitoring of dyestuffs in drinking water, 1990.
No. 228	Measurement of inspirable dyestuff dusts in the dyehouse atmosphere, 1991.

04/04/2009 - ETAD Methods ETAD Toxicological Methods

No. 001*	Determination of the acute oral toxicity (LD₅₀).
No. 002*	Examination for primary skin irritant action.
No. 003*	Examination of irritant action on the eye.
No. 004	Protocol for Salmonella / microsome test (Ames test).
No. 005, 1980	Revised method for Salmonella / microsome test (Ames test).
No. 006	Determination of acute inhalation toxicity of dusts, 1980.
*now superseded by OECD methods.